https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:45137 Wed 26 Oct 2022 13:19:25 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22036 Wed 11 Apr 2018 17:01:12 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:31530 Wed 11 Apr 2018 15:47:21 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:21941 Wed 11 Apr 2018 14:43:38 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22991 Wed 11 Apr 2018 12:51:38 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34354 Tue 12 Mar 2019 16:22:36 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:11216 Sat 24 Mar 2018 08:11:15 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:21471 Gossypium hirsutum) seeds from prefertilization to prestorage phase. GhCWIN1 messenger RNA was abundant at the innermost seed coat cell layer at 5 d after anthesis but became undetectable at 10 d after anthesis, at the onset of its differentiation into transfer cells characterized by wall ingrowths, suggesting that CWIN may negatively regulate transfer cell differentiation. Within the filial tissues, GhCWIN1 transcript was detected in endosperm cells undergoing nuclear division but not in those cells at the cellularization stage, with similar results observed in Arabidopsis (Arabidopsis thaliana) endosperm for CWIN, AtCWIN4. These findings indicate a function of CWIN in nuclear division but not cell wall biosynthesis in endosperm, contrasting to the role proposed for sucrose synthase (Sus). Further analyses revealed a preferential expression pattern of GhCWIN1 and AtCWIN4 in the provascular region of the torpedo embryos in cotton and Arabidopsis seed, respectively, indicating a role of CWIN in vascular initiation. Together, these novel findings provide insights into the roles of CWIN in regulating early seed development spatially and temporally. By comparing with previous studies on Sus expression and in conjunction with the expression of other related genes, we propose models of CWIN- and Sus-mediated regulation of early seed development.]]> Sat 24 Mar 2018 08:03:40 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26958 Solanum lycopersicum) with its CWIN inhibitor gene silenced and focusing on ovaries and fruits at 2 d before and after pollination, respectively. We found that the increase of CWIN activity suppressed LMHS-induced programmed cell death in fruits. Surprisingly, measurement of the contents of H₂O₂ and malondialdehyde and the activities of a cohort of antioxidant enzymes revealed that the CWIN-mediated inhibition on programmed cell death is exerted in a reactive oxygen species-independent manner. Elevation of CWIN activity sustained Suc import into fruits and increased activities of hexokinase and fructokinase in the ovaries in response to LMHS. Compared to the wild type, the CWIN-elevated transgenic plants exhibited higher transcript levels of heat shock protein genes Hsp90 and Hsp100 in ovaries and HspII17.6 in fruits under LMHS, which corresponded to a lower transcript level of a negative auxin responsive factor IAA9 but a higher expression of the auxin biosynthesis gene ToFZY6 in fruits at 2 d after pollination. Collectively, the data indicate that CWIN enhances fruit set under LMHS through suppression of programmed cell death in a reactive oxygen species-independent manner that could involve enhanced Suc import and catabolism, HSP expression, and auxin response and biosynthesis.]]> Sat 24 Mar 2018 07:27:01 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33446 Mon 23 Sep 2019 13:52:05 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:49412 Fri 12 May 2023 14:55:20 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:41582 Fri 05 Aug 2022 14:51:28 AEST ]]>